Intranasal Treatment with Poly(I·C) Protects Aged Mice from Lethal Respiratory Virus Infections
Identifieur interne : 001D01 ( Main/Exploration ); précédent : 001D00; suivant : 001D02Intranasal Treatment with Poly(I·C) Protects Aged Mice from Lethal Respiratory Virus Infections
Auteurs : Jincun Zhao ; Christine Wohlford-Lenane [États-Unis] ; Jingxian Zhao [République populaire de Chine] ; Erica Fleming ; Thomas E. Lane [États-Unis] ; Paul B. Mccray [États-Unis] ; Stanley Perlman [États-Unis]Source :
- Journal of Virology [ 0022-538X ] ; 2012.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Administration par voie nasale, Analyse de survie, Animaux, Charge virale, Cytokines (métabolisme), Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (mortalité), Lipopolysaccharides (administration et posologie), Modèles animaux de maladie humaine, Oligodésoxyribonucléotides (administration et posologie), Poly I-C (administration et posologie), Poumon (anatomopathologie), Poumon (virologie), Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (mortalité), Virus de la grippe A (pathogénicité), Virus du SRAS (pathogénicité).
- MESH :
- administration et posologie : Adjuvants immunologiques, Lipopolysaccharides, Oligodésoxyribonucléotides, Poly I-C.
- anatomopathologie : Poumon.
- mortalité : Infections à Orthomyxoviridae, Syndrome respiratoire aigu sévère.
- métabolisme : Cytokines.
- pathogénicité : Virus de la grippe A, Virus du SRAS.
- virologie : Poumon.
- Administration par voie nasale, Analyse de survie, Animaux, Charge virale, Infections à Orthomyxoviridae, Modèles animaux de maladie humaine, Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Administration, Intranasal, Animals, Cytokines (metabolism), Disease Models, Animal, Influenza A virus (pathogenicity), Lipopolysaccharides (administration & dosage), Lung (pathology), Lung (virology), Mice, Mice, Inbred C57BL, Oligodeoxyribonucleotides (administration & dosage), Orthomyxoviridae Infections (mortality), Orthomyxoviridae Infections (prevention & control), Poly I-C (administration & dosage), SARS Virus (pathogenicity), Severe Acute Respiratory Syndrome (mortality), Severe Acute Respiratory Syndrome (prevention & control), Survival Analysis, Viral Load.
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Lipopolysaccharides, Oligodeoxyribonucleotides, Poly I-C.
- chemical , metabolism : Cytokines.
- mortality : Orthomyxoviridae Infections, Severe Acute Respiratory Syndrome.
- pathogenicity : Influenza A virus, SARS Virus.
- pathology : Lung.
- prevention & control : Orthomyxoviridae Infections, Severe Acute Respiratory Syndrome.
- virology : Lung.
- Administration, Intranasal, Animals, Disease Models, Animal, Mice, Mice, Inbred C57BL, Survival Analysis, Viral Load.
Abstract
In the 2002-2003 severe acute respiratory syndrome coronavirus (SARS-CoV) epidemic, no patients under 24 years of age died, while mortality was greater than 50% in those over 65 years. Greater than 90% of all deaths from influenza A virus (IAV) occur in the elderly (>65 years of age). To address this age-related susceptibility to SARS-CoV and IAV, we infected C57BL/6 (B6) mice with mouse-adapted SARS-CoV (MA15) or IAV (PR8), both of which cause severe disease in aged mice. Intranasal pretreatment of aged mice with poly(I·C) (a TLR3 agonist) and, to a lesser extent, CpG, R848, or lipopolysaccharide (TLR9, TLR7/8, or TLR4 agonists), provided a high level of protection [90% to 100% survival rate after poly(I·C) treatment] against lethal MA15 or IAV challenge and reduced pathological changes and virus loads in the lungs at early times after infection. Poly(I·C) pretreatment upregulated beta interferon (IFN-β), IFN-γ, IL-1β, and tumor necrosis factor (TNF) gene expression in the lungs. Intranasal pretreatment with IFN-β or IFN-γ but not IL-1β or TNF also protected aged mice, consistent with the notion that poly(I·C) pretreatment functioned, at least in part, by inducing IFN-β and IFN-γ. We also identified a potential cellular target for poly(I·C) by showing that treatment inhibited virus replication in primary human airway epithelial cells. These results suggest that intranasal poly(I·C) should be evaluated as a prophylactic agent in aged individuals at high risk for contracting SARS-CoV or IAV infections.
Url:
DOI: 10.1128/JVI.01410-12
PubMed: 22915814
PubMed Central: 3486278
Affiliations:
- République populaire de Chine, États-Unis
- Californie, Guangdong, Iowa
- Iowa City, Jiangmen
- Université de l'Iowa
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000C75
- to stream Pmc, to step Curation: 000C75
- to stream Pmc, to step Checkpoint: 000A52
- to stream PubMed, to step Corpus: 001314
- to stream PubMed, to step Curation: 001314
- to stream PubMed, to step Checkpoint: 001332
- to stream Ncbi, to step Merge: 002554
- to stream Ncbi, to step Curation: 002554
- to stream Ncbi, to step Checkpoint: 002554
- to stream Main, to step Merge: 001D20
- to stream Main, to step Curation: 001D01
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intranasal Treatment with Poly(I·C) Protects Aged Mice from Lethal Respiratory Virus Infections</title><author><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation></author><author><name sortKey="Wohlford Lenane, Christine" sort="Wohlford Lenane, Christine" uniqKey="Wohlford Lenane C" first="Christine" last="Wohlford-Lenane">Christine Wohlford-Lenane</name><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Zhao, Jingxian" sort="Zhao, Jingxian" uniqKey="Zhao J" first="Jingxian" last="Zhao">Jingxian Zhao</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Institute for Tissue Transplantation and Immunology, Jinan University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Tissue Transplantation and Immunology, Jinan University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Fleming, Erica" sort="Fleming, Erica" uniqKey="Fleming E" first="Erica" last="Fleming">Erica Fleming</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation></author><author><name sortKey="Lane, Thomas E" sort="Lane, Thomas E" uniqKey="Lane T" first="Thomas E." last="Lane">Thomas E. Lane</name><affiliation wicri:level="2"><nlm:aff id="aff4">Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Mccray, Paul B" sort="Mccray, Paul B" uniqKey="Mccray P" first="Paul B." last="Mccray">Paul B. Mccray</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22915814</idno><idno type="pmc">3486278</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3486278</idno><idno type="RBID">PMC:3486278</idno><idno type="doi">10.1128/JVI.01410-12</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000C75</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C75</idno><idno type="wicri:Area/Pmc/Curation">000C75</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C75</idno><idno type="wicri:Area/Pmc/Checkpoint">000A52</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A52</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:22915814</idno><idno type="wicri:Area/PubMed/Corpus">001314</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001314</idno><idno type="wicri:Area/PubMed/Curation">001314</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001314</idno><idno type="wicri:Area/PubMed/Checkpoint">001332</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001332</idno><idno type="wicri:Area/Ncbi/Merge">002554</idno><idno type="wicri:Area/Ncbi/Curation">002554</idno><idno type="wicri:Area/Ncbi/Checkpoint">002554</idno><idno type="wicri:doubleKey">0022-538X:2012:Zhao J:intranasal:treatment:with</idno><idno type="wicri:Area/Main/Merge">001D20</idno><idno type="wicri:Area/Main/Curation">001D01</idno><idno type="wicri:Area/Main/Exploration">001D01</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Intranasal Treatment with Poly(I·C) Protects Aged Mice from Lethal Respiratory Virus Infections</title><author><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation></author><author><name sortKey="Wohlford Lenane, Christine" sort="Wohlford Lenane, Christine" uniqKey="Wohlford Lenane C" first="Christine" last="Wohlford-Lenane">Christine Wohlford-Lenane</name><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Zhao, Jingxian" sort="Zhao, Jingxian" uniqKey="Zhao J" first="Jingxian" last="Zhao">Jingxian Zhao</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Institute for Tissue Transplantation and Immunology, Jinan University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Tissue Transplantation and Immunology, Jinan University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Fleming, Erica" sort="Fleming, Erica" uniqKey="Fleming E" first="Erica" last="Fleming">Erica Fleming</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation></author><author><name sortKey="Lane, Thomas E" sort="Lane, Thomas E" uniqKey="Lane T" first="Thomas E." last="Lane">Thomas E. Lane</name><affiliation wicri:level="2"><nlm:aff id="aff4">Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Mccray, Paul B" sort="Mccray, Paul B" uniqKey="Mccray P" first="Paul B." last="Mccray">Paul B. Mccray</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation><nlm:aff id="aff1">Departments of Microbiology</nlm:aff></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Pediatrics, University of Iowa, Iowa City, Iowa, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pediatrics, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term><term>Administration, Intranasal</term><term>Animals</term><term>Cytokines (metabolism)</term><term>Disease Models, Animal</term><term>Influenza A virus (pathogenicity)</term><term>Lipopolysaccharides (administration & dosage)</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Oligodeoxyribonucleotides (administration & dosage)</term><term>Orthomyxoviridae Infections (mortality)</term><term>Orthomyxoviridae Infections (prevention & control)</term><term>Poly I-C (administration & dosage)</term><term>SARS Virus (pathogenicity)</term><term>Severe Acute Respiratory Syndrome (mortality)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Survival Analysis</term><term>Viral Load</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term><term>Administration par voie nasale</term><term>Analyse de survie</term><term>Animaux</term><term>Charge virale</term><term>Cytokines (métabolisme)</term><term>Infections à Orthomyxoviridae ()</term><term>Infections à Orthomyxoviridae (mortalité)</term><term>Lipopolysaccharides (administration et posologie)</term><term>Modèles animaux de maladie humaine</term><term>Oligodésoxyribonucléotides (administration et posologie)</term><term>Poly I-C (administration et posologie)</term><term>Poumon (anatomopathologie)</term><term>Poumon (virologie)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (mortalité)</term><term>Virus de la grippe A (pathogénicité)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Lipopolysaccharides</term><term>Oligodeoxyribonucleotides</term><term>Poly I-C</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term><term>Lipopolysaccharides</term><term>Oligodésoxyribonucléotides</term><term>Poly I-C</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="mortality" xml:lang="en"><term>Orthomyxoviridae Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="mortalité" xml:lang="fr"><term>Infections à Orthomyxoviridae</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Disease Models, Animal</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Survival Analysis</term><term>Viral Load</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie nasale</term><term>Analyse de survie</term><term>Animaux</term><term>Charge virale</term><term>Infections à Orthomyxoviridae</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In the 2002-2003 severe acute respiratory syndrome coronavirus (SARS-CoV) epidemic, no patients under 24 years of age died, while mortality was greater than 50% in those over 65 years. Greater than 90% of all deaths from influenza A virus (IAV) occur in the elderly (>65 years of age). To address this age-related susceptibility to SARS-CoV and IAV, we infected C57BL/6 (B6) mice with mouse-adapted SARS-CoV (MA15) or IAV (PR8), both of which cause severe disease in aged mice. Intranasal pretreatment of aged mice with poly(I·C) (a TLR3 agonist) and, to a lesser extent, CpG, R848, or lipopolysaccharide (TLR9, TLR7/8, or TLR4 agonists), provided a high level of protection [90% to 100% survival rate after poly(I·C) treatment] against lethal MA15 or IAV challenge and reduced pathological changes and virus loads in the lungs at early times after infection. Poly(I·C) pretreatment upregulated beta interferon (IFN-β), IFN-γ, IL-1β, and tumor necrosis factor (TNF) gene expression in the lungs. Intranasal pretreatment with IFN-β or IFN-γ but not IL-1β or TNF also protected aged mice, consistent with the notion that poly(I·C) pretreatment functioned, at least in part, by inducing IFN-β and IFN-γ. We also identified a potential cellular target for poly(I·C) by showing that treatment inhibited virus replication in primary human airway epithelial cells. These results suggest that intranasal poly(I·C) should be evaluated as a prophylactic agent in aged individuals at high risk for contracting SARS-CoV or IAV infections.</p></div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Californie</li><li>Guangdong</li><li>Iowa</li></region><settlement><li>Iowa City</li><li>Jiangmen</li></settlement><orgName><li>Université de l'Iowa</li></orgName></list><tree><noCountry><name sortKey="Fleming, Erica" sort="Fleming, Erica" uniqKey="Fleming E" first="Erica" last="Fleming">Erica Fleming</name><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name></noCountry><country name="États-Unis"><region name="Iowa"><name sortKey="Wohlford Lenane, Christine" sort="Wohlford Lenane, Christine" uniqKey="Wohlford Lenane C" first="Christine" last="Wohlford-Lenane">Christine Wohlford-Lenane</name></region><name sortKey="Lane, Thomas E" sort="Lane, Thomas E" uniqKey="Lane T" first="Thomas E." last="Lane">Thomas E. Lane</name><name sortKey="Mccray, Paul B" sort="Mccray, Paul B" uniqKey="Mccray P" first="Paul B." last="Mccray">Paul B. Mccray</name><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></country><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhao, Jingxian" sort="Zhao, Jingxian" uniqKey="Zhao J" first="Jingxian" last="Zhao">Jingxian Zhao</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D01 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D01 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3486278
|texte= Intranasal Treatment with Poly(I·C) Protects Aged Mice from Lethal Respiratory Virus Infections
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22915814" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |